Human IDO1 ELISA Kit-上海仁捷生物现货

HumanIDO1ELISA Kit

Cat.No.RJ-15030

For the quantitativein vitrodeterminationofHuman indoleamine 2,3-dioxygenase 1concentrations in

serum - plasma - tissue homogenates - other biological fluids

FOR LABORATORY RESEARCH USE ONLY.

NOT FOR USE IN DIAGNOSTIC PROCEDURES.

This package insert must be read in its entirety before using thisproduct.

ELISA

ENZYME LINKED IMMUNOSORBENT ASSAY

INTENDEDUSEAND TEST PRINCIPLE

ThisIDO1ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofIDO1in the sample, thisIDO1ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusIDO1concentration. The concentration ofIDO1in the samples is then determined by comparing the O.D. of the samples to the standard curve.

SAMPLE COLLECTION AND STORAGES

Serum- Use a serum separator tube and allow samples to clot for two hours at room temperature or overnight at4℃before centrifugation for 20 minutes at approximay 1000×g. Assay freshly prepared serumimmediay or store samples in aliquot at -20℃or -80℃for later use. Avoid repeated freeze/thaw cycles.

Plasma- Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at1000×g at 2-8℃within 30 minutes of collection. Remove plasma and assay immediay or store samplesin aliquot at -20℃or -80℃for later use. Avoid repeated freeze/thaw cycles.

Tissue homogenates- For general information, hemolysis blood may affect the result, so you shouldrinse the tissues with ice-cold PBS (0.01M, pH=7.4) to remove excess blood thoroughly. Tissuepieces should be weighed and then minced to small pieces which will be homogenized in PBS (thevolume depends on the weight of the tissue. 9mL PBS would be appropriate to 1 gram tissue pieces.Some protease inhibitor is recommended to add into the PBS.) with a glass homogenizer on ice. Tofurther break the cells, you can sonicate the suspension with an ultrasonic cell disrupter or subject it tofreeze-thaw cycles. The homogenates are then centrifugated for 5minutes at 5000×g to get thesupernate.

Cell culture supernatesand other biological fluids -Centrifuge samples for 20 minutes at 1000×g. Removeparticulates and assay immediay or store samples in aliquot at -20℃or -80℃for later use. Avoid repeatedfreeze/thaw cycles.

Note:The samples shoule be centrifugated dequay and no hemolysis or granule was allowed.

MATERIALSREQUIREDBUTNOT SUPPLIED

1.37 ℃ incubator

2.Standard microplate readercapableofmeasuringabsorbanceat450nm

3.Precision pipettes, disposable pipette tipsandAbsorbent paper

4.Distilled or deionized water

REAGENTS PROVIDED

All reagents provided are stored at 2-8°C. Refer to the expiration date on the label.

Name	96determinations	48determinations
MICROTITERPLATE	8*12strips	8*6strips
STANDARD（6 vial）	0.3ml/vial	0.3ml/vial
SAMPLE DILUENT	6.0ml	3.0ml
ENZYME CONJUGATE	10.0ml	5.0ml
WASH SOLUTION	25ml	15ml
SUBSTRATEA	6.0ml	3.0ml
SUBSTRATEB	6.0ml	3.0ml
STOP SOLUTION	6.0ml	3.0ml
Closure plate membrane	2	2
User manual	1	1
Sealed bags	1	1

Note:

1.Standard concentrationwas followed by:24, 12, 6, 3, 1.5, 0.75IU/mL.

2.If samples generate values higher than the highest standard,please dilute thesamples with Sample Diluent and repeat the assay.

PRECAUTIONS

• Do not substitute reagents from one kit lot to another. Standard, conjugate and microtiter plates are matched for optimal performance. Use only the reagents supplied by manufacturer.
• Allow kit reagents and materials to reach room temperature (20-25°C) before use. Do not use water baths to thaw samples or reagents.
• Do not use kit components beyond their expiration date.
• Use only deionized or distilled water to dilute reagents.
• Do not remove microtiter plate from the storage bag until needed. Unused strips should be stored at 2-8°C in their pouch with the desiccant provided.
• Use fresh disposable pipette tips for each transfer to avoid contamination.
• Do not mix acid and sodium hypochlorite solutions.
• Serum and plasma should be handled as potentially hazardous and capable of transmitting disease. Disposable gloves must be worn during the assay procedure, since no known test method can offer complete assurance that products derived fromRatblood will not transmit infectious agents. Therefore, all blood derivatives should be considered potentially infectious and good laboratory practices should be followed.
• All samples should be disposed of in a manner that will inactivate viruses.
• Liquid Waste: Add sodium hypochlorite to a final concentration of 1.0%. The waste should be allowed to stand for a minimum of 30 minutes to inactivate the viruses before disposal.
• Substrate Solution is easily contaminated. If bluish prior to use,do not use.
• Substrate B contain20% acetone, keep this reagent away from sources of heat or flame.
• Remove all kit reagents from refrigerator and allow them to reach room temperature ( 20-25°C).

REAGENT PREPARATION ANDSTORAGE

WashSolution(1X) -Dilute 1 volume of Washsolution(20X) with19volumes of deionized or distilled water. WashSolutionis stable for 1 month at 2-8°C.